Difco manual mannitol salt agar

The nasal swabs were streaked on mannitol salt agar (Difco, Detroit, MI) and incubated at 37°C for 24 h. Colonies with suggestive morphology of staphylococci that also fermented mannitol were submitted to Gram staining, catalase, coagulase, DNase tests, and PCR amplification of femB Susceptibility tests were performed by disk diffusion (Oxoid Ltd., Basingstoke, Hampshire, . Difco ™ Malt Extract Broth BAM Cat. No. Dehydrated – g *Store at °C. Mannitol Salt Agar Intended Use This medium conforms with specifications of The United States Pharmacopeia (USP). Mannitol Salt Agar is used for the selective isolation and enu-meration of staphylococci from clinical and nonclinical materials. Summary and. Mannitol salt agar is available in dehydrated powder form by various manufacturers like Mast, Oxoid, Difco, Himedia, and so on. Follow the instructions of the manufacturer to prepare the medium i.e. g dehydrated medium inmL distilled water, mix properly and finally Sterilize by autoclaving at °C for 15 minutes.

Acid production from mannitol was observed by a change in colour of the mannitol-salt agar plate. Haemolysis of colonies and yellow pigment were observed on trypticase soy agar II with 5 % sheep. of your lab manual) Mannitol salt • Mannitol salt agar is a selective and differential medium used for differentiating between different stapylococci. recommends Mannitol Salt Agar as a test medium for isolating Mannitol Salt Agar - DILACO The Manual of Microbiological Culture Media is a valuable resource for all laboratories using dehydrated, bottled, tubed and plated media. BD Difco™ BBL™ Manual (Second Edition) includes a CD with an electronic copy for easy access and searching.

Mannitol Salt Agar. Difco BBL Manual, 2nd Edition 9. Mannitol Salt Agar. Liofilchem SRL. BD Mannitol Salt Agar. Becton Dickinson GmbH. Provide the requested information using a difco manual or a BBL manual a. c) Percentage of sodium chloride in mannitol salt agar. Mannitol Salt Agar (MSA) is used as a selective and differential medium for This medium is also included in the Bacteriological Analytical Manual for.